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CD16 Mouse anti-Baboon, Human, Rhesus, Brilliant Ultraviolet 737, Clone: 3G8, BD Horizon™

Mouse Monoclonal Antibody

Marque:  BD Biosciences 612786

Informations supplémentaires : Poids : 0.00060kg

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Code produit. 17257884

  • 638.00€ / 100 tests
Expédition estimée: 14-10-2022
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Description

Description

The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (Fc γRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al ., 1997) to increase intracellular calcium levels in human neutrophils by interacting with both Fc γRIIa and Fc γRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation.

The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).

Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (e.g., CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

Spécification

Spécification

CD16
Monoclonal
Brilliant Ultraviolet 737
Mouse
Affinity Purified
RUO
Rhesus Monkey, Human, Baboon
IgG1 κ
Flow Cytometry
3G8
Aqueous buffered solution containing ≤0.09% sodium azide.
Human polymorphonuclear leukocytes
100 Tests
Primary
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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