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CD19 Monoclonal Antibody (HIB19), NovaFluor™ Yellow 610, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Marque: Invitrogen H004T03Y03
Informations supplémentaires : Poids : 0.02000kg
Description
The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.
Applications Reported: This HIB19 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 4 μL (0.6 μg) per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
When using more than one NovaFluor dye, CellBlox Monocyte and Macrophage Blocking Buffer may be used to block non-specific binding of dyes to monocytes.
Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm (Yellow) Laser
Spécification
| CD19 | |
| Monoclonal | |
| 5 μL/Test | |
| PBS with BSA and 0.09% sodium azide; pH 7.2 | |
| P15391 | |
| Cd19 | |
| Affinity chromatography | |
| RUO | |
| 930 | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| HIB19 | |
| NovaFluor Yellow 610 | |
| Cd19 | |
| AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12 | |
| Mouse | |
| 100 Tests | |
| Primary | |
| Human | |
| Antibody | |
| IgG1 κ |