CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor™ Blue 660-120S, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Marque: Invitrogen H001T02B08
Informations supplémentaires : Poids : 0.02000kg
The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and at low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.
Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 2 μL (0.1 μg) per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
When using more than one NovaFluor dye, CellBlox Monocyte and Macrophage Blocking Buffer may be used to block non-specific binding of dyes to monocytes.
Excitation: 509 nm; Emission: 665 nm; Laser: 488 nm (Blue) Laser
|PBS with no preservative; pH 7.2|
|NovaFluor Blue 660-120S|
|CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 receptor; CD4, allele 1; cd4a; CD4mut; cell surface glycoprotein CD4; fCD4; L3T4; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); W3/25; W3/25 antigen|
|4° C, store in dark, DO NOT FREEZE!|