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CD64 (Fc gamma Receptor 1) Mouse anti-Human, Super Bright 600, Clone: 10.1, eBioscience™

Mouse Monoclonal Antibody

Marque:  Invitrogen 63-0649-42

Informations supplémentaires : Poids : 0.01000kg

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Code produit. 15993514

  • 330.00€ / 100 pièces
Expédition estimée: 29-07-2022
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Description

Description

Description: The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 is the high affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative. Applications Reported: The 10.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 10.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or contact eBioscience Technical Support (tech@ebioscience.com) for more information.

Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.
Spécification

Spécification

CD64 (Fc gamma Receptor 1)
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P12314
Mouse
100 Tests
Primary
Human
Liquid
Flow Cytometry
10.1
Super Bright 600
FCGR1A
FCGR1A
Affinity chromatography
RUO
2209
Antibody
IgG1 κ
Vidéos
FDS
Documents
Certificats
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