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CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Super Bright™ 436, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
179.00€ - 418.00€
Spécification
Antigène | CD95 (APO-1/Fas) |
---|---|
Clone | 15A7 |
Concentration | 0.2 mg/mL |
Applications | Flow Cytometry |
Classification | Monoclonal |
Code produit | Marque | Quantité | Prix | Quantité et disponibilité | |||||
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Code produit | Marque | Quantité | Prix | Quantité et disponibilité | |||||
15855503
|
Affymetrix eBioscience
62-0951-82 |
100 μg |
418.00€
100µg |
Expédition estimée: 24-06-2024 Connectez-vous pour voir le stock disponible |
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15845503
|
Affymetrix eBioscience
62-0951-80 |
25 μg |
Cet article est actuellement indisponible ou discontinu.
Veuillez consulter la page produit pour de possible alternative dans l’onglet |
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Description
Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells. Applications Reported: This 15A7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.Spécification
CD95 (APO-1/Fas) | |
0.2 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
P25446 | |
FAS | |
Primary | |
4° C, store in dark, DO NOT FREEZE! |
15A7 | |
Flow Cytometry | |
Super Bright 436 | |
Mouse | |
Mouse | |
14102 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only