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IgM Rat anti-Mouse, PerCP-eFluor™ 710, Clone: II/41, eBioscience™

Rat Monoclonal Antibody

Marque:  Affymetrix eBioscience 46-5790-80

Code nomenclature Nacres: NA.41

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15341350

  • 110.00€ / 25µg

Expédition estimée
23-10-2020

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Description

Description

Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Spécification

Spécification

IgM
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
II/41
PerCP-eFluor™ 710
Ighm
Ighm
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16019
Vidéos
Documents

For Research Use Only.