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Invitrogen™ IL-6 Porcine ELISA Kit
Sandwich ELISA Kit
702.00€ - 2635.00€
Spécification
Numéro d’adhésion | P26893 |
---|---|
Sensibilité du dosage | 45 pg/ml |
Doser | ELISA sandwich en phase solide |
Conjugué | Biotine |
Méthode de détection | Colorimétrique |
Code produit | Marque | Quantité | Prix | Quantité et disponibilité | |||||
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Code produit | Marque | Quantité | Prix | Quantité et disponibilité | |||||
15445403
|
Invitrogen™
ESIL6 |
1/boÙte |
702.00€
1 kit |
En stock
Connectez-vous pour voir le stock disponible |
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17145979
|
Invitrogen™
ESIL6X5 |
5 x 96 tests |
2635.00€
1 pièce |
Expédition estimée: 31-05-2024
Connectez-vous pour voir le stock disponible |
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Description
The Porcine Interleukin 6 (IL-6) ELISA quantitates Porc IL-6 in porcine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porc IL-6. Principle of the method The Porcine IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.Spécification
P26893 | |
ELISA sandwich en phase solide | |
Colorimétrique | |
Cytokines & récepteurs | |
La paire d’anticorps ELISA sandwich détecte l’IL-6 d’origine porcine. | |
Lecteur de microplaques colorimétriques | |
IL6 | |
<10% | |
HRP | |
Immunologie | |
2°C à 8°C | |
1 heures 20 min |
45 pg/ml | |
Biotine | |
Kit ELISA | |
Plasma, Sérum, Surnageant | |
ELISA, dosage des protéines, biologie des protéines, cancérologie, immunologie, neuroscience, neurobiologie, détection des protéines, analyses protéiques | |
399500 | |
<12% | |
Solution d’arrêt, Plaque(s) pré-enduite(s), étalon ou calibrateur, anticorps détecteur, conjugué HRP, diluants, tampon de lavage, substrat(s) et solution d’arrêt, Tampon de lavage, Anticorps de détection biotinylé, SAV-HRP, Concentrat de diluant de dosage, Plaque à 96 puits pré-enduite, Couvercles de plaques adhésives, Étalon, Chromogène | |
RUO | |
Plasma, 50 μl ; sérum, 50 μl ; surnageant, 100 μl | |
Porcs | |
4 heures 45 min |