p16INK4a Monoclonal Antibody (1D7D2), Invitrogen™

Description

MA5-17054 targets CDKN2A in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human samples. The MA5-17054 immunogen is purified recombinant fragment of human CDKN2A (amino acids: 1-156) expressed in E. Coli. MA5-17054 detects CDKN2A which has a predicted molecular weight of approximately 16.5kDa.

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.