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Rat IgG1 kappa, Super Bright 702, eBioscience™

Rat Isotype Control

Marque:  Invitrogen 67-4301-82

Informations supplémentaires : Poids : 0.01000kg

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Code produit. 15993754

  • 310.00€ / 100µg
Expédition estimée: 26-07-2022
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Description

Description

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis and ELISA. Applications Tested: This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension, This can be used at the same concentration as the experimental antibody. Excitation: 405nm; Emission: 702nm; Laser: Violet Laser.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Spécification

Spécification

Rat IgG1 kappa
eBRG1
Super Bright 702
Rat
100 μg
Control
Liquid
Control, Flow Cytometry
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
Affinity chromatography
RUO
Not Applicable
IgG1 κ
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