VAP-1 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Seq. of rec. protein: GRGGDGGEPS QLPHCPSVSP SAQPWTHPGQ SQLFADLSRE ELTAVMRFLT QRLGPGLVDA AQARPSDNCV FSVELQLPPK AAALAHLDRG SPPPAREALA IVFFGRQPQP NVSELVVGPL PHPSYMRDVT VERHGGPLPY HRRPVLFQEY LDIDQMIFNR ELPQASGLLH HCCFYKHRGR NLVTMTTAPR GLQSGDRATW FGLYYNISGA GFFLHHVGLE LLVNHKALDP ARWTIQKVFY QGRYYDSLAQ LEAQFEAGLV NVVLIPDNGT GGSWSLKSPV PPGPAPPLQF YPQGPRFSVQ GSRVASSLWT FSFGLGAFSG PRIFDVRFQG ERLVYEISLQ EALAIYGGNS PAAMTTRYVD GGFGMGKYTT PLTRGVDCPY LATYVDWHFL LESQAPKTIR DAFCVFEQNQ GLPLRRHHSD LYSHYFGGLA ETVLVVRSMS TLLNYDYVWD TVFHPSGAIE IRFYATGYIS SAFLFGATGK YGNQVSEHTL GTVHTHSAHF KVDLDVAGLE NWVWAEDMVF VPMAVPWSPE HQLQRLQVTR KLLEMEEQAA FLVGSATPRY LYLASNHSNK WGHPRGYRIQ MLSFAGEPLP QNSSMARGFS WERYQLAVTQ RKEEEPSSSS VFNQNDPWAP TVDFSDFINN ETIAGKDLVA WVTAGFLHIP HAEDIPNTVT VGNGVGFFLR PYNFFDEDPS FYSADSIYFR GDQDAGACEV NPLACLPQAA ACAPDLPAFS HGGFSHN. Sandwich ELISA: To detect Hu VAP-1 by sandwich ELISA (using 100 uL/well), a conc. of 0.5-2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Rabbit Anti-Hu VAP-1 (500-P326BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of rec. Hu VAP-1. WB: To detect Hu VAP-1 by WB analysis this antibody can be used at a conc. of 0.1-0.2 ug/mL. When used in conjunction with compatible secondary reagents, the detection limit for rec. Hu VAP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.