Électrophorèse, Western blot et ELISA

Thermo Scientific™ Échelle de protéines précolorée PageRuler™ Plus 10-250kDa

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X10 PAGERULER PLUS PRES PROT LADLAD

Thermo Scientific™ Échelle de protéines précolorée PageRuler™ 10-180 kDa

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X10 PAGERULER PREST PROTEIN LADDERLADDER

Invitrogen™ Gels Bolt™ Bis-Tris Plus à 4-12 %, 10 puits

Gels de polyacrylamide prémoulés conçus pour une séparation optimale des protéines de petite et moyenne dimension dans des conditions dénaturantes X10 Bolt 4-12 percent Bis-Tris Plus Gels, 10-well

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Thermo Scientific™ Colorant de charge d’ADN 6X

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Tampon de migration Novex™ NuPAGE™ MOPS SDS (20X)

Fabriqué avec des réactifs de grande pureté et soumis à un contrôle qualité strict NUPAGE MOPS 20XRUN BUF

Invitrogen™ Novex™ SeeBlue™ Plus2 Pre-stained Protein Standard

Allows you to easily visualize protein molecular weight ranges during electrophoresis and quickly evaluate Western transfer efficiency SEEBLUE PLUS2

Invitrogen™ Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0mm, 10 well

Pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions NUPAGE 4-12% BT GEL 1.0MM10W

Invitrogen™ Agent réducteur d’échantillons Novex™ NuPAGE™ (10X)

Réactif prêt à l’emploi pour réduire les échantillons protéiques pour l’électrophorèse sur gel des protéines. NP SAMPLE RED AGT 10X

Thermo Scientific™ Tampon de déshybridation de transfert Western Restore™

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. RESTORE WESTERN BLOT STRIPPING BUFBUF

Invitrogen™ Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0mm, 12 well

Pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions NUPAGE 4-12% BT GEL 1.0MM12W

Thermo Scientific™ Substrat chimiluminescent SuperSignal™ West Femto

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis. SUPERSIGNAL WEST FEMTO CHEMILUMINESCENT SUBSTRATETrial kit, SuperSignal 20mL Store in Fridge at 4C

Invitrogen™ Novex™ NativeMARK™ Unstained Protein Standard, Unstained

Ready-to-use protein standard designed for molecular weight estimation of proteins using native gel electrophoresis NATIVEMARK UNSTD PROTEIN STD

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis SUPERSIGNAL WEST PICO PLUS

Invitrogen™ Empilements de transfert en nitrate de cellulose Novex™ iBlot™ 2

Utilisés pour transférer des protéines avec le dispositif de transfert de gel iBlot 2 10 stacks, iBlot 2 Transfer Stacks, nitrocellulosenitrocellulose, mini

Invitrogen™ DYNAL™ MyOne™ Dynabeads™ Streptavidin C1

Exceptional standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets DYNABEADS? MYONE STREPTAVIDINFor Research Use Only. All usage must comply with

Invitrogen™ Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5mm, 10 well

Pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions X10 NUPAGE 4-12% BT GEL 1.5MM

Invitrogen™ Gels Bolt™ Bis-Tris Plus à 4-12 %, 15 puits

Gels de polyacrylamide prémoulés conçus pour une séparation optimale des protéines de petite et moyenne dimension dans des conditions dénaturantes X10 Bolt 4-12 percent Bis-Tris Plus Gels, 15-well

Invitrogen™ Antioxydant Novex™ NuPAGE™

Réactif exclusif qui conserve les protéines à l’état réduit pendant l’électrophorèse sur gel des protéines. 15ML NUPAGE ANTIOXIDANT

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels NUPAGE TA SDS RNBUF (20X)

Acrylamide: Bis-Acrylamide 29:1 (40% Solution/Electrophoresis), Fisher BioReagents

1LT Acrylamide:Bis-Acrylamide 29:1 solution 40%, DNase and RNase free, electrophoresis tested

Invitrogen™ Novex™ SimplyBlue™SafeStain

Less hazardous and does not require methanol or acetic acid fixatives or destains SIMPLYBLUE 3.5L

Invitrogen™ Novex™ Sharp Pre-stained Protein Standard

Designed for accurate, easy, and convenient molecular weight estimation of a wide range of molecular weight proteins during SDS-PAGE and Western blotting NOVEX SHARP PS PROT STD

Thermo Scientific™ Tampon de déshybridation de transfert Western Restore™ Plus

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. RESTORE PLUS WESTERN BLOT 30ML

Agarose (faible EEO/polyvalent/qualité biologie moléculaire), Fisher BioReagents

N° CAS: 9012-36-6 Formule moléculaire: C12H18O9 500GR Agarose, Low-EEO/Multi-Purpose, Molecular Biology Grade

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Thermo Scientific™ Réactif de coloration bleue GelCode™

Achieve nanogram-level detection and results that exceed those of typical coomassie stains, all without a destaining step. 3.5 LT Blue stain reagent, GelCode 3.5L Store in

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines eBioscience Foxp3 StainingBuffer Set

Thermo Scientific™ Substrats de TMB Pierce™

Substrats de péroxydase de raifort (HRP) pour ELISA qui passent du bleu au jaune lors de l’ajout d’une solution d’arrêt à l’acide sulfurique ou phosphorique. 1-STEP SLOW TMB SUBSTRATE 250ML250ML

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