Invitrogen™ CD51 (Integrin alpha V) Monoclonal Antibody (RMV-7), eBioscience™, Invitrogen™

Description

Description: The RMV-7 monoclonal antibody reacts with the mouse CD51 molecule, the integrin alpha v chain. This approximately 120 kDa surface molecule non-covalently associates with the beta subunits of the integrin family including beta3 (CD61), beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by platelets, T cells and granulocytes and mediate adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin. Applications Reported: The RMV-7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and functional assays. Soluble RMV-7 blocks adhesion to fibronectin and vitronectin and immobilized RMV-7 induces activation. (Please use Functional Grade purified RMV-7, cat. 16-0512, in functional assays.). Applications Tested: The RMV-7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8, 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands, the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.